Western blot proficiency course – visionofresearch

Western blot proficiency course

In many molecular and diagnostic laboratories, it is necessary to separate certain proteins. One of the most widely used methods for isolating and identifying the target protein from the collection of tissue or blood proteins is the western blot method. With the help of this method, in a relatively short time, the investigated protein can be separated from the collection of cellular proteins, and its concentration can also be roughly estimated. Among the applications of Western Blot technique, it is possible to mention the confirmation of gene expression during an investigated process and the detection of many viral infections.

  • Information
  • Course Type : Biomedical
  • Start Course : 1404/06/10
  • Method : Online

  • Language :
  • Capacity : Person
  • Countdown Timer
Duration

2 Month

Number Of Session

36 Sessions Session

Days of holding

Monday

Time

10:58:19

Researchers who are working in biology research, especially research projects of biology, cellular and molecular trends, as well as people who are looking for work in the molecular department of medical diagnostic laboratories, can be easily attracted to the job market with the help of acquiring this skill.

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Western blots allow the resolution of proteins on SDS-PAGE as a function of their molecular weights, followed by their transfer on an adsorbent membrane. The subsequent addition of antibody probes allows the detection of specific proteins on the membrane.

The Western blot test separates the blood proteins and detects the specific proteins (called HIV antibodies) that indicate an HIV infection. The Western blot is used to confirm a positive ELISA, and the combined tests are 99.9% accurate.
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